(Part 2) Molecular characterization of <i>Mycobacterium ulcerans</i> DNA gyrase and identification of mutations reduced susceptibility to quinolones <i>in vitro</i>

Buruli ulcer disease is a neglected necrotizing and disabling cutaneous tropical illness caused by Mycobacterium ulcerans (Mul). Fluoroquinolone (FQ), used in the treatment of this disease, has been known to act inhibiting enzymatic activities DNA gyrase; however, detailed molecular basis these characteristics FQ resistance mechanisms Mul remains unknown. In study investigated mechanism gyrase contribution vitro using recombinant proteins from subsp. shinshuense Agy99 strains with reduced sensitivity FQs. Furthermore, interaction between amino acid residues WT/mutant side chains was assessed via docking studies. This first elaborative demonstrating mutations GyrA subunit vitro.